This is your main asset as an actor and you want to make sure it's showcasing your acting skills in the best possible light. Points to consider:
Does this reel highlight your acting at its best?
Could you record or edit any footage to strengthen it?
Does this still look like you? (are you using old/out-of-date footage?)
Does this reel consist of work you are passionate about? (Think of your branding and what you are putting out for consideration.)
Length. Is my reel too long and not keeping the viewer engaged?
Are you using footage just because you think you have to? (you paid for a reel you spent lots of money on, or a scene from drama school.)
Is the production value letting you down? It may appear as though you are an amateur.
Just be sure your reel is the very best it can be.
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Great questions for actors to ask themselves, Tammy Hunt! What's the ideal length for a reel?
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Really great points here. I wanted to piggyback and note that including your one line on NCIS is totally acceptable, but you probably don't need to use your one line from a student film. The reason is that the NCIS clip, though not really showcasing your ability, is signaling to casting that you have been on a big set before and that other CDs or producers have seen enough of your work to feel comfortable putting you on set with stars. Each piece of content should either showcase what you can do (like a really meaty scene in a student film, indie, or a scene you paid to produce), or show what you have done ("look, I was on set with James Spader in The Blacklist where I had two lines. Others producers put me on set, and you can too."). If one piece of content does both, even better.